Journal: Experimental & molecular medicine

Article Title: Identification of Escherichia coli 8-oxoguanine endonuclease.

doi: 10.1038/emm.2000.26

Figure Lengend Snippet: Figure 1. Chromatographic purification of E. coli oh8Gua endonuclease. After removal of nucleic acids with streptomycin, crude extract was separated by first phenyl HPLC (A), DEAE HPLC (B), gel filtration chromatography (C), second phenyl HPLC (D) and heparin affinity HPLC (E). For activity assay, 5 or 10 µl of each fraction was incubated with 5’ end-labeled substrate DNA containing oh8Gua. DNA nick at the site of oh8Gua was visualized with sequencing gel electrophoresis and autoradiography. For collection of active fractions, percentage of cleavage was determined by densitometry. Solid line, dashed line and dotted solid line indicate absorbance at 280 nm (protein profile; arbitrary unit except gel filtration chromatography), salt concentration and activity (% of nick), respectively.

Article Snippet: To determine the collection of active fractions, % of DNA breakage was estimated by densitometry (Model 620, BioRad, USA).

Techniques: Purification, Filtration, Chromatography, Activity Assay, Incubation, Labeling, Sequencing, Nucleic Acid Electrophoresis, Autoradiography, Concentration Assay

Journal: Scientific Reports

Article Title: Immunoproteomic Identification and Characterization of Leishmania Membrane Proteins as Non-Invasive Diagnostic Candidates for Clinical Visceral Leishmaniasis

doi: 10.1038/s41598-018-30546-y

Figure Lengend Snippet: (A) Two-dimensional gel electrophoresis of Leishmania membrane proteins (LAg). 51, 55 and 63 kDa spots were picked and subjected to tryptic digestion. (B) 63, 55 and 51 kDa proteins of LAg were electroeluted and subjected to SDS-PAGE. Corresponding lanes in part B was part of a single gel.

Article Snippet: Excised protein bands were eluted using electrophoresis apparatus (BioRad, Model-422) under running buffer (1% SDS, 0.192 M glycine and 0.025 M Tris) for 4 h at 10 mA.

Techniques: Two-Dimensional Gel Electrophoresis, Electrophoresis, Membrane, SDS Page